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ecgm2 medium  (PromoCell)
96
PromoCell ecgm2 medium
A. Merged bright field and GFP fluorescence microscopy images of spheroids composed of HepaRG, GFP-HUVEC, and MSC cells at a ratio of 5:2:2 that were cultured in <t>ECGM2</t> 2%FCS, EH 2%FCS, or EH 4%SR for 4, 7, or 14 days. B. Projected area of spheroids cultured in ECGM2 2%FCS, EH 2%FCS, or EH 4%SR on days 4, 7, and 14 after cell seeding. A graphical representation with individual data points is shown in Supplemental Figure S1. (C, D). Numbers of Ki-67-positive (C) and E-cadherin-positive (D) cells within spheroids cultured in ECGM2 2%FCS, EH 2%FCS, or EH 4%SR on day 7 after seeding. E. Representative Ki-67 and E-cadherin immunofluorescence images of paraffin sections of spheroids cultured in ECGM2 2%FCS, EH 2%FCS, or EH 4%SR on day 7 after cell seeding. 4′,6-diamidino-2-phenylindole (DAPI) staining marks the cell nuclei. F. Representative fluorescence microscopy images showing GFP-positive vessel-like structures within spheroids cultured in EH 2%FCS or EH 4%SR for 7 days. Means ± SEM are shown. Scale bars: 100 µm. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, n.s. – non-significant.
Ecgm2 Medium, supplied by PromoCell, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ecgm2 medium/product/PromoCell
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ecgm2  (InvivoGen)
96
InvivoGen ecgm2
A. Merged bright field and GFP fluorescence microscopy images of spheroids composed of HepaRG, GFP-HUVEC, and MSC cells at a ratio of 5:2:2 that were cultured in <t>ECGM2</t> 2%FCS, EH 2%FCS, or EH 4%SR for 4, 7, or 14 days. B. Projected area of spheroids cultured in ECGM2 2%FCS, EH 2%FCS, or EH 4%SR on days 4, 7, and 14 after cell seeding. A graphical representation with individual data points is shown in Supplemental Figure S1. (C, D). Numbers of Ki-67-positive (C) and E-cadherin-positive (D) cells within spheroids cultured in ECGM2 2%FCS, EH 2%FCS, or EH 4%SR on day 7 after seeding. E. Representative Ki-67 and E-cadherin immunofluorescence images of paraffin sections of spheroids cultured in ECGM2 2%FCS, EH 2%FCS, or EH 4%SR on day 7 after cell seeding. 4′,6-diamidino-2-phenylindole (DAPI) staining marks the cell nuclei. F. Representative fluorescence microscopy images showing GFP-positive vessel-like structures within spheroids cultured in EH 2%FCS or EH 4%SR for 7 days. Means ± SEM are shown. Scale bars: 100 µm. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, n.s. – non-significant.
Ecgm2, supplied by InvivoGen, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ecgm2/product/InvivoGen
Average 96 stars, based on 1 article reviews
ecgm2 - by Bioz Stars, 2026-02
96/100 stars
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complete growth medium ecgm2  (PromoCell)
99
PromoCell complete growth medium ecgm2
A. Merged bright field and GFP fluorescence microscopy images of spheroids composed of HepaRG, GFP-HUVEC, and MSC cells at a ratio of 5:2:2 that were cultured in <t>ECGM2</t> 2%FCS, EH 2%FCS, or EH 4%SR for 4, 7, or 14 days. B. Projected area of spheroids cultured in ECGM2 2%FCS, EH 2%FCS, or EH 4%SR on days 4, 7, and 14 after cell seeding. A graphical representation with individual data points is shown in Supplemental Figure S1. (C, D). Numbers of Ki-67-positive (C) and E-cadherin-positive (D) cells within spheroids cultured in ECGM2 2%FCS, EH 2%FCS, or EH 4%SR on day 7 after seeding. E. Representative Ki-67 and E-cadherin immunofluorescence images of paraffin sections of spheroids cultured in ECGM2 2%FCS, EH 2%FCS, or EH 4%SR on day 7 after cell seeding. 4′,6-diamidino-2-phenylindole (DAPI) staining marks the cell nuclei. F. Representative fluorescence microscopy images showing GFP-positive vessel-like structures within spheroids cultured in EH 2%FCS or EH 4%SR for 7 days. Means ± SEM are shown. Scale bars: 100 µm. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, n.s. – non-significant.
Complete Growth Medium Ecgm2, supplied by PromoCell, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
complete growth medium ecgm2 - by Bioz Stars, 2026-02
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50ul well complete ecgm2  (PromoCell)
99
PromoCell 50ul well complete ecgm2
A. Merged bright field and GFP fluorescence microscopy images of spheroids composed of HepaRG, GFP-HUVEC, and MSC cells at a ratio of 5:2:2 that were cultured in <t>ECGM2</t> 2%FCS, EH 2%FCS, or EH 4%SR for 4, 7, or 14 days. B. Projected area of spheroids cultured in ECGM2 2%FCS, EH 2%FCS, or EH 4%SR on days 4, 7, and 14 after cell seeding. A graphical representation with individual data points is shown in Supplemental Figure S1. (C, D). Numbers of Ki-67-positive (C) and E-cadherin-positive (D) cells within spheroids cultured in ECGM2 2%FCS, EH 2%FCS, or EH 4%SR on day 7 after seeding. E. Representative Ki-67 and E-cadherin immunofluorescence images of paraffin sections of spheroids cultured in ECGM2 2%FCS, EH 2%FCS, or EH 4%SR on day 7 after cell seeding. 4′,6-diamidino-2-phenylindole (DAPI) staining marks the cell nuclei. F. Representative fluorescence microscopy images showing GFP-positive vessel-like structures within spheroids cultured in EH 2%FCS or EH 4%SR for 7 days. Means ± SEM are shown. Scale bars: 100 µm. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, n.s. – non-significant.
50ul Well Complete Ecgm2, supplied by PromoCell, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
50ul well complete ecgm2 - by Bioz Stars, 2026-02
99/100 stars
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ecgm2 medium  (InvivoGen)
96
InvivoGen ecgm2 medium
A. Merged bright field and GFP fluorescence microscopy images of spheroids composed of HepaRG, GFP-HUVEC, and MSC cells at a ratio of 5:2:2 that were cultured in <t>ECGM2</t> 2%FCS, EH 2%FCS, or EH 4%SR for 4, 7, or 14 days. B. Projected area of spheroids cultured in ECGM2 2%FCS, EH 2%FCS, or EH 4%SR on days 4, 7, and 14 after cell seeding. A graphical representation with individual data points is shown in Supplemental Figure S1. (C, D). Numbers of Ki-67-positive (C) and E-cadherin-positive (D) cells within spheroids cultured in ECGM2 2%FCS, EH 2%FCS, or EH 4%SR on day 7 after seeding. E. Representative Ki-67 and E-cadherin immunofluorescence images of paraffin sections of spheroids cultured in ECGM2 2%FCS, EH 2%FCS, or EH 4%SR on day 7 after cell seeding. 4′,6-diamidino-2-phenylindole (DAPI) staining marks the cell nuclei. F. Representative fluorescence microscopy images showing GFP-positive vessel-like structures within spheroids cultured in EH 2%FCS or EH 4%SR for 7 days. Means ± SEM are shown. Scale bars: 100 µm. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, n.s. – non-significant.
Ecgm2 Medium, supplied by InvivoGen, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ecgm2 medium/product/InvivoGen
Average 96 stars, based on 1 article reviews
ecgm2 medium - by Bioz Stars, 2026-02
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endothelial cell growth medium 2 ecgm2 promocell  (PromoCell)
99
PromoCell endothelial cell growth medium 2 ecgm2 promocell
A. Merged bright field and GFP fluorescence microscopy images of spheroids composed of HepaRG, GFP-HUVEC, and MSC cells at a ratio of 5:2:2 that were cultured in <t>ECGM2</t> 2%FCS, EH 2%FCS, or EH 4%SR for 4, 7, or 14 days. B. Projected area of spheroids cultured in ECGM2 2%FCS, EH 2%FCS, or EH 4%SR on days 4, 7, and 14 after cell seeding. A graphical representation with individual data points is shown in Supplemental Figure S1. (C, D). Numbers of Ki-67-positive (C) and E-cadherin-positive (D) cells within spheroids cultured in ECGM2 2%FCS, EH 2%FCS, or EH 4%SR on day 7 after seeding. E. Representative Ki-67 and E-cadherin immunofluorescence images of paraffin sections of spheroids cultured in ECGM2 2%FCS, EH 2%FCS, or EH 4%SR on day 7 after cell seeding. 4′,6-diamidino-2-phenylindole (DAPI) staining marks the cell nuclei. F. Representative fluorescence microscopy images showing GFP-positive vessel-like structures within spheroids cultured in EH 2%FCS or EH 4%SR for 7 days. Means ± SEM are shown. Scale bars: 100 µm. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, n.s. – non-significant.
Endothelial Cell Growth Medium 2 Ecgm2 Promocell, supplied by PromoCell, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/endothelial cell growth medium 2 ecgm2 promocell/product/PromoCell
Average 99 stars, based on 1 article reviews
endothelial cell growth medium 2 ecgm2 promocell - by Bioz Stars, 2026-02
99/100 stars
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A. Merged bright field and GFP fluorescence microscopy images of spheroids composed of HepaRG, GFP-HUVEC, and MSC cells at a ratio of 5:2:2 that were cultured in ECGM2 2%FCS, EH 2%FCS, or EH 4%SR for 4, 7, or 14 days. B. Projected area of spheroids cultured in ECGM2 2%FCS, EH 2%FCS, or EH 4%SR on days 4, 7, and 14 after cell seeding. A graphical representation with individual data points is shown in Supplemental Figure S1. (C, D). Numbers of Ki-67-positive (C) and E-cadherin-positive (D) cells within spheroids cultured in ECGM2 2%FCS, EH 2%FCS, or EH 4%SR on day 7 after seeding. E. Representative Ki-67 and E-cadherin immunofluorescence images of paraffin sections of spheroids cultured in ECGM2 2%FCS, EH 2%FCS, or EH 4%SR on day 7 after cell seeding. 4′,6-diamidino-2-phenylindole (DAPI) staining marks the cell nuclei. F. Representative fluorescence microscopy images showing GFP-positive vessel-like structures within spheroids cultured in EH 2%FCS or EH 4%SR for 7 days. Means ± SEM are shown. Scale bars: 100 µm. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, n.s. – non-significant.

Journal: bioRxiv

Article Title: Self-organized vascularized human liver spheroids: Serum-free culture conditions and use as tissue building blocks

doi: 10.1101/2025.10.25.684548

Figure Lengend Snippet: A. Merged bright field and GFP fluorescence microscopy images of spheroids composed of HepaRG, GFP-HUVEC, and MSC cells at a ratio of 5:2:2 that were cultured in ECGM2 2%FCS, EH 2%FCS, or EH 4%SR for 4, 7, or 14 days. B. Projected area of spheroids cultured in ECGM2 2%FCS, EH 2%FCS, or EH 4%SR on days 4, 7, and 14 after cell seeding. A graphical representation with individual data points is shown in Supplemental Figure S1. (C, D). Numbers of Ki-67-positive (C) and E-cadherin-positive (D) cells within spheroids cultured in ECGM2 2%FCS, EH 2%FCS, or EH 4%SR on day 7 after seeding. E. Representative Ki-67 and E-cadherin immunofluorescence images of paraffin sections of spheroids cultured in ECGM2 2%FCS, EH 2%FCS, or EH 4%SR on day 7 after cell seeding. 4′,6-diamidino-2-phenylindole (DAPI) staining marks the cell nuclei. F. Representative fluorescence microscopy images showing GFP-positive vessel-like structures within spheroids cultured in EH 2%FCS or EH 4%SR for 7 days. Means ± SEM are shown. Scale bars: 100 µm. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, n.s. – non-significant.

Article Snippet: 2.5 mg/ml collagen type I (C3867-1VL, Sigma-Aldrich) was neutralized with ∼9% v/v ice-cold 0.2 N NaOH (1091371000, Merck, Darmstadt, Germany) and 1x Medium 199 (M0650-100ML, Sigma-Aldrich), and mixed at a 1:1 ratio with spheroids in 1.2% methylcellulose (M0512-100G, Sigma-Aldrich, viscosity: 4,000 cP), prepared as described in Tetzlaff et al, 2018 [ ] in ECGM2 medium (C-22211, PromoCell).

Techniques: Fluorescence, Microscopy, Cell Culture, Immunofluorescence, Staining